Exploring Eluent pH in Method Development

Introduction

Exploring the Power of Eluent pH in LC Method Development
Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in analyte retention from eluent pH can be helpful for screening systems and method development platforms where observing the number of components in samples is important before method optimisation is attempted. The chromatographic selectivity 'space' for various eluent and column permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical hints and tips for ensuring repeatable and reproducible chromatography, when working with an extended pH range, will be included.

Exploring chromatographic selectivity
Stationary phase design
Acidic and basic analyte ionisation theory
Choosing appropriate stationary phases
HPLC and UHPLC
Practical hints and tips

Available as in-house or off-site courses. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk

Deadlines

Sep 23 2016
Standard registration deadline

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Venue

Trinity Centre Cambridge

Suite 1, Trinity Centre Cambridge, 24 Cambridge Science Park, Milton Road, Cambridge, CB4 0FN, United Kingdom

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Hichrom is one of the world’s leading HPLC column manufacturers and has been firmly established in the field of Liquid Chromatography for over 30 years, developing, manufacturing and supplying the highest quality LC columns throughout the world. During this time Hichrom has built up a strong reputation amongst chromatographers for our technical expertise. Our speakers are experienced chromatographers who will be happy to discuss your questions on a wide variety of HPLC/UHPLC topics including: troubleshooting, method development, reproducibility, batch reservation, custom packing/bonding, application support, method transfer & column selection.

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