We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and quantify the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of Enrichment based sequencing results using standard Illumina software tools like MiSeq Reporter and VariantStudio.
EMBL Course: Next Generation Sequencing: Enrichment Based Targeted Resequencing
6 - 10 February 2017, Heidelberg, Germany
Introduction
Venue
EMBL ATC
EMBL ATC, Meyerhofstr 1, Meyerhofstr 1, Heidelberg, 69117, Germany
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