HPLC Instrument Basics and Troubleshooting
This short course is suitable for all analysts working in HPLC. Predominately focussing on the HPLC instrument, it covers the essential concepts and operational principles of High Performance Liquid Chromatography. The course will look at the various modules of an HPLC instrument and will consider briefly how these work and what can go wrong with them. By understanding the problems that commonly occur, analysts will be in a better position to identify solutions and resolve or prevent HPLC method problems.
• Anatomy of an HPLC Instrument
• Temperature
• Mobile phases, buffers and pH
• Detectors
• Pumps and mixing
• Valves, fittings, tubing & loops
• Summary and conclusions
This short course is suitable for all analysts working in HPLC. Predominately focussing on the HPLC instrument, it covers the essential concepts and operational principles of High Performance Liquid Chromatography. The course will look at the various modules of an HPLC instrument and will consider briefly how these work and what can go wrong with them. By understanding the problems that commonly occur, analysts will be in a better position to identify solutions and resolve or prevent HPLC method problems.
• Anatomy of an HPLC Instrument
• Temperature
• Mobile phases, buffers and pH
• Detectors
• Pumps and mixing
• Valves, fittings, tubing & loops
• Summary and conclusions
